光斑的中心提取：重心法和圆拟合。先用多次迭代求阈值并且对图像二值化，再用重心法和圆拟合提取光斑中心。-light spot method：median point ，circle fitting

光斑质心计算，可以完成光斑质心点的确定，可同时计算36质心点-Spot centroid calculation can be done to determine the centroid of the light spot, which can calculate the centroid 36

用于共焦显微系统中虚拟针孔的设定，包含图像预处理，光斑定位，灰度值相加和绘制图像功能-Confocal microscopy system for virtual pinhole settings including image preprocessing, spot location, value addition and gray image rendering function