Run the ROI file by issuing the command ROI in the command window and a GUI screen will pop up, the screen may be used for selecting a Region of interest while viewing the full length movie and adjusting the region of interest accordingly, after hitting save the GUI will dismantle the movie to seperate images and will save them in current folder.The GUI panel is self explanatory and as such does not require any further explanations-Run the ROI file by issuing the command ROI in the command window and a GUI screen will pop up, the screen may be used for selecting a Region of interest while viewing the full length movie and adjusting the region of interest accordingly, after hitting　save　the GUI will dismantle the movie to seperate images and will save them in current folder.The GUI panel is self explanatory and as such does not require any further explanations