When the two nucleic acid sequences, when compared, K-Estimator used to assess the number of nucleotide substitutions between the two (Divergent), including the protein coding region and non-protein coding region. Using Monte Carlo simulation estimates of confidence intervals. In molecular evolutionary studies to assess the two nucleic acid sequences is replaced by the number of nucleotides is a central issue. Accurate quantification of these data will directly affect the evolution of genetics is widely used in many experiments of reliability.