：针对显微图像分析、识别需要的全自动控制显微镜，研究开发其相应的图像处理算法。提

出了能量谱自动聚焦评价函数算法、自适应选取基准图的图像拼接算法、改进 Laplacian算子的多层

聚焦图像叠合算法等。该算法模块已成功地应用于所开发的CMIAS 显微医学图像分析系统中，取得

了满意的应用效果。-【ABSTRACT】For the automatic microscope　required by　micro-image　analysis and　recognition, the

relevant image processing a lgorithms are investigated.　Three algorithms are described: energy-spectrum

image auto focusing evaluation function, image merging based on the adaptive selection of standard image　

and　sum-modified-Laplacian (SML) operator for multi-focus image fusion.　The algorithms have been

applied successfully to CMIAS　medical micro-optical image　analysis　system and testified to be feasible

and effective.